| 1. | Gfp expression was monitored using a fluorescence microscope . the result showed that the fusion gene was expressed at a low level
利用脂质体转染的方法在美国棉铃虫细胞hz中表达了gfp - actin融合基因。 |
| 2. | After flushed the fluorescence intensity difference between treatment group and control group was observed under fluorescence microscope . 4
冲洗后在荧光倒置显微镜下观察实验组和对照组巨噬细胞的荧光强弱差异。 |
| 3. | After flushing the fluorescence intensity difference between treatment group and control group was observed under fluorescence microscope
冲洗后激光扫描共聚焦显微镜下观察实验组和对照组巨噬细胞的荧光强弱的差异。 |
| 4. | The changes of the expression of gfp in biu - 87 cell that induced by the aconitine and hab toxins , gtx were detected with fluorescence microscope , and quantitatively measured with image - pro plus software
经诱导，抗性细胞发出较强的绿色荧光，表明重组质粒pegfp - c - fos在biu - 87细胞中成功表达。 |
| 5. | The expression of scfvs fusion protein were detectable by fluorescence microscope directly and indirect immunofluorescence and immunohistochemistry analysis after transient expression in cos - 7
瞬时转染cos刁细胞后，通过荧光显微镜观察、间接免疫荧光检测、免疫组化检测证实了scfv融合蛋白的表达。 |
| 6. | Identification area : the main equipment here is an ordinary micro - scope , on fluorescence microscope , incubator , instrument for bacte - ria identification , low temperature or ultra low temperature refrigera - tor ( for the temporary storage of bacteria shall follow the regulations of the cdc )
3鉴定区：主要有普通显微镜、荧光显微镜、温箱、细菌鉴定仪、低温或超低温冰箱（菌种临时保存，视cdc规定而定）等。 |
| 7. | Equipment and instruments : electronic analytic balance , uv and vis spectrophotometer , 97rt fluorescence spectrophotometer , gas chromatograph spectrometer , high speed centrifugal machines , leica rm 2015 microtome , fluorescence microscopes , pcr amplifier , and so on
:电子分析天平、紫外可见光分光光度计、 97rt荧光分光光度计、气相色谱仪（附4种检测器） 、高速离心机、病理切片机、荧光显微镜、 pcr扩增仪等。 |
| 8. | Sf9 cells was infected by these two recombinant virus . the expression of gfp - actin fusion gene and gfp gene were checked with the fluorescence microscope and through sds - page . the distribution of fluorescence in the progress of the infection was checked under the confocal scanning light microscope
用这两种重组病毒分别感染sf9细胞，以acmnpv - gfp感染的sf9细胞为对照，用普通荧光显微镜和sds - page的方法分析了融合基因gfp - actin和gfp基因的表达情况。 |
| 9. | After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells , the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope , indirect immunofluorescence and flow cytometer analysis . prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed
建系细胞培养上清与肝癌细胞作用后，经荧光显微镜观察、间接免疫荧光及流式细胞仪检测进一步确定表达的scfv融合蛋白具有与hbsag特异性结合的活性。 |
| 10. | To observe tsarg2 fusion protein expression in mammalian cell , pegfp - nl / tsarg2 fusion plasmid was constructed and transiently introduced into cos7 cell by liposome transfection . under the fluorescence microscope , the green fluorescence produced by pegfp - nl / rsarg2 was detected on the nucleus of cos7 cells after 24 hours post transfection , while the fluorescence produced by pegfp - nl was detected through the cells
同时将21大、 35天、 49大、 280天小鼠睾丸进行组织石蜡切片，结果表明小鼠翠丸在第21天可见精子细胞，到第35天，精子生成的最后阶段开始发生，部分牛精小管中可见少量成熟的精于。 |
